|
KMID : 0620920080400010071
|
|
Experimental & Molecular Medicine
2008 Volume.40 No. 1 p.71 ~ p.83
|
|
|
Nur77 upregulates HIF-α by inhibiting pVHL-mediated degradation
|
|
Kim Bu-Yeon
Cho Eun-Jung
Kim Hyung-Soo
Youn Hong-Duk
|
|
|
Abstract
|
|
|
|
In this study, we investigated the role of Nur77, an orphan nuclear receptor, in HIF-α transcriptional activity. We found that Nur77 associates and stabilizes HIF-1α via indirect interaction. Nur77 was found to interact with pVHL in vivo via the α-domain of pVHL. By binding to pVHL, Nur77 competed with elongin C for pVHL binding. Moreover, Nur77-binding to pVHL inhibited the pVHL-mediated ubiquitination of HIF-1α and ultimately increased the stability and transcriptional activity of HIF-1α. The ligand-binding domain of Nur77 was found to interact with pVHL and the expression of this ligand-binding domain was sufficient to stabilize and transactivate HIF-1α. Under the conditions that cobalt chloride was treated or pVHL was knocked down, Nur77 could not stabilize HIF-α. Moreover, Nur77 could not further stabilize HIF-2α in A498/VHL stable cells, which is consistent with our finding that Nur77 indirectly stabilizes HIF-α by binding to pVHL. Thus, our results suggest that an orphan nuclear receptor Nur77 binds to pVHL, thereby stabilizes and increases HIF-α transcriptional activity under the nonhypoxic conditions.
|
|
|
KEYWORD
|
|
|
hypoxia-inducible factor 1, alpha subunit, orphan nuclear receptor NGFI-B, ubiquitination, , VHL protein, human
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
    
|
|